The following grades were scored in six points around each tooth on maxillary and mandibular anterior region: Twenty interdental units were identified, giving a maximum overgrowth score of for a patient. Gingival overgrowth was assessed on these individual models by performing measurements in vertical and horizontal dimensions.
The vertical and horizontal scores were calculated to determine an overgrowth score for each gingival papillae. In another animal study, Gau et al. However, when all drug groups were considered, saliva nitrite and nitrate levels could not be used as a biomarker for drug-induced gingival overgrowth.
Drug-induced gingival overgrowth has a multifactorial nature and the pathogenesis is still uncertain. Citric acid was applied to the dorsum of the tongue, held there by the subject for 10 s and 1 ml of saliva was collected from each patient as described in detail before Francis et al.
The maximum overgrowth score obtained for each interdental unit was five. Nitrite and nitrate content of the samples extracts was determined using Griess reagent Grisham et al.
Although NO metabolites have a very short life, nitrite and nitrate are the relatively stable end products of NO oxidation Lappin et al. Clinical parameters Periodontal examinations were carried out on the upper and lower six anterior teeth by one calibrated periodontal specialist ES during the study period.
GCF samples were obtained from maxillary anterior teeth.
Only patients receiving one of these drugs including CsA, phenytoin, nifedipine, and diltiazem in regular doses for at least 6 months were included in the study.
Materials and methods Study population and design This research study was conducted at the Department of Periodontology, Hacettepe University between February and September Therefore, the present study was conducted to evaluate nitrite and nitrate levels in plasma, saliva, and GCF from responders and non-responders treated with either CsA, phenytoin, nifedipine, or diltiazem, and to determine whether NO levels in plasma, saliva, and GCF can serve as a potential biomarker for the evaluation of drug-induced gingival overgrowth risk.
It has been shown that NO takes part in the etiopathogenesis of many diseases, including periodontal disease Ohashi et al. Due to the fact that NO levels could be affected by smoking, smokers were not included in the study.
Patients participated in this study were obtained from outpatients attending the Department of Nephrology, Neurology, and Cardiology, Faculty of Medicine, Hacettepe University.Full-Text Paper (PDF): Determination of Nitrite Levels in Maize Samples From Different Regions of Turkey.
obtained had a high level of accuracy and repeatability with a relative standard deviation of %.
Note: If nitrite concentration is required rather than nitrite-nitrogen, multiply the calculated nitrite-nitrogen Water Analysis Using LAMBDA Nitrite Determination Application Note Keywords. Dec 01, · To the best of our knowledge, this is the first study that has evaluated the association of plasma, saliva, and GCF nitrite/nitrate levels with drug-induced gingival overgrowth in patients receiving either CsA, phenytoin, nifedipine, or diltiazem therapy.
hazards, most international organizations set legal limits to nitrite content in drinking water. The European Union limit for drinking water is mg L-1 of NO N. For this reason, nitrite levels are routinely determined in quality control analyses of drinking, waste, marine and underground waters, among others.
Determination of the Levels of Nitrite in Homemade Brews, Spirits Homemade brews and the raw materials used can be a major source of nitrate and nitrite in human diet. Let us write you a custom essay sample on ##customtitle##.
Prepared by the U.S. Geological Survey Office of Water Quality, National Water Quality Laboratory Colorimetric Determination of Nitrate Plus Nitrite in.Download